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Table 1 Mutagenesis primers for the hemagglutinin gene

From: Glycosylation at 11Asn on hemagglutinin of H5N1 influenza virus contributes to its biological characteristics

Mutation

Direction

Primer sequence (5′-3′)

SY S10A

Forward

CATGCAAACAACGCGACAGA

Reverse

TCTGTCGCGTTGTTTGCATG

SY T11A

Forward

CATGCAAACAACTCGGCAGA

Reverse

TCTGCCGAGTTGTTTGCATG

SY ST10/11AA

Forward

CATGCAAACAACGCGGCAGA

Reverse

TCTGCCGCGTTGTTTGCATG

  1. The substitution nucleotides are underlined.
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Veterinary Research

ISSN: 1297-9716