Figure 4

Ad5-F and Ad5-H vaccination activates T cell responses to epitopes induced in PPRV infections. The IFN-γ production to A F and B H epitopes was assessed in Ad5-F- and Ad5-H-vaccinated sheep, respectively by ELISPOT assays. The PBMC used in this experiment were from Ad5-F or Ad5-H immunized sheep obtained 21 days after booster vaccination and prior to PPRV challenge. Data are presented as mean IFN-γ spots normalized to control for each sheep. Inactivated PPRV IC’89 was used as positive control. The dotted horizontal line represents the positive stimulation threshold (positive counts > 10 and at least 2 standard deviations above background; control well spot counts < 25). C–F Intracellular IFN-γ staining and flow cytometry analysis used to identify T cell subsets responding to F and H epitopes in PBMC expanded with peptide for 1 week. Representative dot-plots of CD4⁺ T cell responses to C F epitopes and control (no peptide) in Ad5-F-vaccinated sheep and D H epitopes and control (no peptide) in Ad5-H-vaccinated sheep. Representative dot-plots of CD8⁺ T cell responses to E F epitopes and control (no peptide) in Ad5-F-vaccinated sheep and F H epitopes and control (no peptide) in Ad5-H-vaccinated sheep.