Establishment of an intestinal crypt culture system. Crypts from mouse small intestines were seeded into Matrigel and cultured for 72 h to obtain well-developed organoids. A Kinetics of the growth of a single isolated crypt. B Cell counts of a single organoid cultured for 4 days. C Single isolated crypts efficiently form large crypt organoids within 4 days, along with several surrounding crypt-like domains. Mean values ± SE of triplicate assays are shown. Bars: 20 μm (A); 50 μm (C).