Characterization of JOL1950 ghosts constructed by endolysin expression. A Western blot analysis. Endolysin expressed in JOL1950 was confirmed by rabbit anti-endolysin polyclonal antibodies. Black arrows indicate the expected size of endolysin (~17 kDa). Lane M, size marker; lane 1, JOL1950 grown in the presence of l-arabinose at 27 °C; lane 2, JOL1950 ghosts produced under lysis conditions. Characterization of JOL1950 S. Typhimurium ghost cells by scanning electron microscopy (SEM). B Intact JOL1950 cells before lysis. C JOL1950 ghost cells after 36 h of lysis. Arrowheads indicate lysis transmembrane tunnels.