Table 2 Distribution of CZ and JY viruses and their recombinants with single or double substitutions in the PB2 in mouse organs at 3 and 5 dpi
Virus | Virus titer (log10EID50/mL ± SD) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Heart | Liver | Spleen | Lung | Kidney | Brain | |||||||
3 dpi | 5 dpi | 3 dpi | 5 dpi | 3 dpi | 5 dpi | 3 dpi | 5 dpi | 3 dpi | 5 dpi | 3 dpi | 5 dpi | |
r-CZ | 1.88 ± 0.53 | 2.88 ± 0.53 |  | 2.50 ± 0.00 | 2.63 ± 0.18 | 1.93 ± 0.13 | 5.88 ± 0.53 | 6.40 ± 0.50 | 1.50 ± 0.35 | 2.00 ± 0.71 | 2.00 ± 0.35 | 5.25 ± 0.00 |
CZPB2-M283I | – | – | – | – | 2.63 ± 0.18 | 2.00 ± 0.35 | 4.13 ± 0.58 | 3.38 ± 0.18 | – | – | – | – |
CZPB2-R526K | – | 2.03 ± 0.03 |  | – | 3.13 ± 0.53 | 2.88 ± 0.53 | 5.88 ± 0.53 | 5.88 ± 0.53 | 1.25 ± 0.00 | 1.88 ± 0.18 | – | 2.50 ± 0.35 |
CZPB2-M283I-R526K | – | – | – | – | 1.38 ± 0.53 | 1.50 ± 0.00 | 4.63 ± 0.18 | 2.88 ± 0.88 | – | – | – | – |
r-JY | – | – | – | – | – | – | 3.63 ± 0.13 | 4.13 ± 0.38 | – | – | – | – |
JYPB2-I283M | – | – | – | – | – | – | 4.63 ± 0.18 | 4.88 ± 0.53 | – | – | – | – |
JYPB2-P321S | – | – | – | – | 1.25 ± 0.00 | – | 4.63 ± 0.18 | 4.13 ± 0.63 | – | – | 1.38 ± 0.18 | – |
JYPB2-K526R | – | 1.75 ± 0.00 | – | – | – | – | 4.50 ± 0.00 | 4.88 ± 0.53 | – | – | – | – |
JYPB2-V529I | – | – | – | – | – | – | 3.38 ± 0.18 | 3.63 ± 0.18 | – | – | – | – |
JYPB2-I283M-K526R | – | 2.00 ± 0.35 | – | – | 1.38 ± 0.18 | – | 6.13 ± 0.53 | 4.50 ± 0.71 | – | 1.50 ± 0.35 | – | – |