Protecting effect of PrP codons M142 and K222 in goats orally challenged with bovine spongiform encephalopathy prions

Table 2 Data concerning the effectiveness of the oral challenges in goats with bovine (1 ^st passage) and caprine (2 ^nd passage) BSE

Genotype	Numbers			Numbers from 12 mpi				Inc. time clinical, mpi ±SD (n)
Genotype	Total	Before 12 mpi	After 12 mpi	Pre-clinical	Late preclinical	Clinical	Attack ratios affected/total (%) + significance^a	Inc. time clinical, mpi ±SD (n)
bovBSE
wt	24	6	18	3	2	5	10/18 (56%)	25.2 ± 0.4 (5)
I/M142	10	2	8	0	0	2	2/8 (25%)**	44.5 ± 0.7 (2)
M/M142	4	0	4	0	1	0	1/4 (25%)	NA
R/Q211	12	3	9	0	5	3	8/9 (89%)	30.7 ± 2.1 (3)
Q/K222	12	3	9	0	1^b	0	1/9 (11%)**	NA
gtBSE
wt	26	6	20	2	3	5	10/20 (50%)	25.6 ± 1.5 (5)
I/M142	11	3	8	0	0	0	0/8 (0%)*	NA
R/Q211	11	4	7	1	0	3	4/7 (57%)	34.3 ± 1.5 (3)
Q/K222	11	3	8	0	1^c	0	1/8 (13%)^c*	NA

Genotype	Total	mpi			Numbers from 12 mpi			Inc. time clinical, mpi ±SD (n)
Genotype	Total	1^st clin case	Last clin case	Pre-clinical	Late preclinical	Clinical	Attack ratios affected/total (%) + significance	Inc. time clinical, mpi ±SD (n)
Combined challenges
wt	50	24	28	5	5	10	20/38 (53%)	25.4 ± 1.1 (10)
I/M142	21	44	45	0	0	2	2/16 (13%)**	44.5 ± 0.7 (2)
M/M142	4	NA	NA	NA	NA	NA	NA	NA
R/Q211	23	28	36	1	5	6	12/16 (75%)	32.5 ± 2.6 (6)
Q/K222	23	43	NA	0	2	0	2/17 (12%)***	NA

NA, not applicable.
^aStatistical data: compared to the wild type group. Fisher’s exact test, *, **, and *** respectively p < 0.05, p < 0.01, p < 0.001.
^bThis Q/K222 goat was CNS- and muscle-positive.
^cOne Q/K222 case was CNS-positive, and psoas-muscle-positive but by mouse bioassay (Tg110) only [33]. If considered negative (by IHC) this group was statistically positive compared to the wild type group (p < 0.05).

ISSN: 1297-9716