Figure 3

PrP ^D accumulation in central and peripheral nervous tissues of BSE infected goats. A Brain stem of a clinical goat BSE infected wt/wt goat, 24 mpi, showing a severe intra- and extracellular PrP^D accumulation. Antibody 6C2, bar 50 µm. B Brain stem of the late preclinical cattle BSE infected Q/K222 goat, 43 mpi, showing a severe intra- and extracellular PrP^D accumulation in the obex (DMNV). Antibody Bar224, bar 20 µm. C Spinal nerve of a clinical cattle BSE infected wt/wt goat, 25 mpi, showing moderate intra-axonal and intraglial PrP^D accumulation. Antibody Bar224, bar 100 µm. D Ileum of a clinical cattle BSE infected wt/wt goat, 25 mpi, showing PrP^D accumulation in neurons and glial cells of the enteric nervous system as well as intracellular in lymphoid cells of the ileal follicle. Antibody Bar224, bar 20 µm.