Figure 4
From: The role of phospholipase C signaling in bovine herpesvirus 1 infection
Effect of U73122 and edelfosine on Erk1/2 and p38MAPK signaling in response to BoHV-1 infection. Serum starved MDBK cells in 60-mm dishes were pretreated with U73122 (A) or edelfosine (B) at the indicated concentrations for 1 h, respectively, then infected with BoHV-1 (MOI = 10) in the presence of respective inhibitors for 0.5 h. (C) Serum starved MDBK cells in 60-mm dishes were exposed to DMSO, U73122 or edelfosine at the indicated concentrations for 1.5 h. Cell lysate was prepared and subjected to western blotting analysis. The band intensity was analyzed with software image J. The data are representatives of three independent experiments.