Figure 1

Inhibitory effect of U73122 and edelfosine on BoHV-1 viral replication in vitro. Inhibitory effect of U73122 (A), edelfosine (B) and ACY (C) on BoHV-1 replication in MDBK cells. Serum starved MDBK cells were pretreated with each inhibitor at indicated concentrations for 1 h, infected with BoHV-1(MOI = 1) for 24 h along with the treatment of corresponding inhibitor, respectively. The viral titer was determined with MDBK cells with the results expressed as TCID₅₀/mL. Inhibitory effect of U73122 (D), edelfosine (E) and ACY (F) on BoHV-1 replication in RS-1 cells. Serum starved RS-1 cells were pretreated with each inhibitor at the indicated concentrations for 1 h, infected with BoHV-1(MOI = 1) for 24 h along with the treatment of the corresponding inhibitor, respectively. The viral titer was determined with MDBK cells with results expressed as TCID₅₀/mL. (G) The cytotoxicity assay for both U73122 and edelfosine in either MDBK cells or RS-1 cells. MDBK cells or RS-1 cells were exposed DMSO, U73122 or edelfosine at the designated concentrations for 24 h. After incubation with MTT, the OD value was determined and compared to the control which was designated as 100%. Data represent three independent experiments. Significance was assessed with the student t test (*P < 0.05).