Figure 6

Inflammatory response during BTV infection. A CD14⁺ cells were quantified by flow cytometry from total PBMC obtained from BTV-infected sheep. B RNA was extracted from PBMC from BTV-infected sheep at different times post-infection. Reverse transcription to cDNA and IL-6, IL-1β, IL-12 and CXCL10 expression was assessed by RT-qPCR. The line represents the average gene expression in 4–8 animals tested at each time point. The arrows indicate time of infection with BTV-8. Data were normalized to β-actin expression and to pre-infection values (t = 0) (2^−∆∆Ct method). Student t test (time point vs t = 0); * p ≤ 0.05, ** p ≤ 0.01.