Table 2 Primers and siRNA used in this study
Name | 5′ → 3′ |
|---|---|
Degenerated primers | |
CH-F1 | ATCTCWGCHGAYAGYGCCATCAT |
CH-F2 | ATCAGYGSDGAGACCATHTTTGT |
CH-F3 | GCYAAGATCTACATYGACAGCA |
CH-F4 | AGGAAGCHAARCTIACHGACCA |
CH-R1 | CTCTGYYTCCAGCGGTTGTT |
CH-R2 | GAGITAYTCYCTCATGACITGYATR |
CH-R3 | CAGGCAGGCBGCAAARCACT |
CL-F1 | GATTTTGACATGCTGAACGC |
CL-F2 | ATTGAAAACGACGAAGGCTT |
CL-F3 | AATGGAGAGCTTCATGGGGA |
CL-R1 | TTCAGGGAGATGAGGACCGA |
CL-R2 | CCTGCTTGCTGGACTTGGG |
Race primers | |
CHR5-R1 | CTGGGTTTCTGACAGTGCT |
CHR5-R2 | CCTGGTCACACTGTCCTCTCTC |
CHR3-F1 | TCAACTTCTTCAGCAAGGTG |
CHR3-F2 | ACTATCAGGCACTGAGGACCTC |
CLR5-R1 | TTCTTGGCCCAGCAAGAGAGC |
CLR5-R2 | CCGCTGGAAACGGTGTCGG |
CLR3-F1 | AACCCCAAGTCCAGCAAGCAG |
CLR3-F2 | CATGCGCTCCGTCCTCATCT |
siRNA sequence | |
CHC1-S | GUUAAGGAGGCCAUUGACUCC |
CHC1-A | AGUCAAUGGCCUCCUUAACCA |
CHC2-S | GUACAGAACCACAACAACAAA |
CHC2-A | UGUUGUUGUGGUUCUGUACUG |
CHC3-S | GCAUUUAAUUAUUGUAUAUUC |
CHC3-A | AUAUACAAUAAUUAAAUGCAU |
CLC1-S | GGUACUUUCUAUAACUACUAC |
CLC1-A | AGUAGUUAUAGAAAGUACCUG |
CLC2-S | GCUUAUGCAGCAAUUUCCAAU |
CLC2-A | UGGAAAUUGCUGCAUAAGCAU |
CLC3-S | GUUUGUAUGUGUGUUUAAACC |
CLC3-A | UUUAAACACACAUACAAACGG |
IR1-S | AAUAGUCAUUGUGUAUGGCCA |
IR1-A | GCCAUACACAAUGACUAUUUG |
IR2-S | AAUGGCUAAGGUUUUGAUCAC |
IR2-A | GAUCAAAACCUUAGCCAUUAU |