Table 1 Antibody panels
Antigen | Clone | Isotype | Fluorochrome | Labelling strategy | Source of primary Ab |
|---|---|---|---|---|---|
Intracellular cytokine staining for IL-17A and TNF-α | |||||
 CD4 | 74-12-4 | IgG2b | PerCP-Cy5.5 | Directly conjugated | BD Biosciences |
 CD8α | 11/295/33 | IgG2a | Pe-Cy7 | Secondary antibodya | In house |
 TCR-γδ | PPT16 | IgG2b | BV421 | Biotin–streptavidinb | In house |
 IL-17A | SCPL1362 | IgG1 | Alexa647 | Directly conjugated | BD Biosciences |
 TNF-α | MAb11 | IgG1 | BV605 | Directly conjugated | BioLegend |
Intracellular cytokine staining for IL-10 | |||||
 CD4 | 74-12-4 | IgG2b | Alexa647 | Secondary antibodyc | In house |
 CD8α | 11/295/33 | IgG2a | BV421 | Biotin–streptavidinb | In house |
IL-10 | 945A 4C4 37B1 | IgG1 | PE | Secondary antibodyd | Invitrogen |