Table 1 List of the genes selected for the quantitative real time RT-PCR validation
Gene name | Sequence of the primers | Ensembl ID | Primer position | Description |
|---|---|---|---|---|
GBP2 | F: CCTGTGGTGGTGGTGGTTAT | ENSSSCG00000006923 | Exon 3–4 | Guanylate binding protein 2, interferon-inducible |
R: AGATGCCCTTCGTGTGAGAC | ||||
IL1A | F: CAAGGACAGTGTGGTGATGG | ENSSSCG00000008090 | Exon 4–5 | Interleukin 1, alpha |
R: GTTGCTGATCTGGGCTTGAT | ||||
IL8 | F: CCTTCTTGGCAGTTTTCCTG | ENSSSCG00000008953 | Exon 1–2 | Interleukin 8 |
R: AATTTGGGGTGGAAAGGTGT | ||||
CCL4 | F: CATGAAGCTCTGCGTGACTG | ENSSSCT00000019264 | Exon 1–2 | Chemokine (C–C motif) ligand 4 |
R: ACGGTGTATGTGAAGCAGCA | ||||
SAA1 | F: ACTATGATGCTGCCCAAAGG | ENSSSCT00000014601 | Exon 1–2 | Serum amyloid A1 |
R: ACTCCGTGGCCACTGTCTC | ||||
IGF1 | F: AGTTCGTGTGCGGAGACAG | ENSSSCT00000000935 | Exon 2–3 | Insulin-like growth factor 1 |
R: GCCTCCTCAGATCACAGCTC | ||||
CCL2 | F: CTTCTGCACCCAGGTCCTT | ENSSSCT00000019290 | Exon 1–2 | Chemokine (C–C motif) ligand 2 |
R: TGCTGCTGGTGACTCTTCTG | ||||
IL1B | F: AGTGGAGAAGCCGATGAAGA | ENSSSCT00000008861 | Exon 4–5 | Interleukin 1, beta |
R: CATTGCACGTTTCAAGGATG | ||||
LTBP1 | F: GGGAACACCACCACTCTCAT | ENSSSCT00000009313 | Exon 1–2 | Latent transforming growth factor beta binding protein 1 |
R:TTGTCCCTTGAACTGCACTG |