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Figure 1 | Veterinary Research

Figure 1

From: Distinct functional enrichment of transcriptional signatures in pigs with high and low IFN-gamma responses after vaccination with a porcine reproductive and respiratory syndrome virus (PRRSV)

Figure 1

Scheme of the study design. Firstly, pigs were distributed in two groups: intramuscularly (IM) inoculated either with Pyrsvac-183 vaccine (n = 22) or sterile solution (SS) as unvaccinated control group (n = 10). Animals were followed-up from day 0 (pre-vaccination) to 6 weeks post-vaccination (PV). Blood and sera samples were collected every week for immunological determinations (ELISPOT virus-specific IFN-γ secreting cells -SC-, neutralizing antibodies -NA- assays, ELISA total Ab). At week 6 PV, vaccinated pigs with the highest and lowest average scores for the IFN-γ-SC were divided into the High Responder (HR) and the Low Responder (LR) groups, respectively. At this last time point, gene expression was analysed in PBMCs from the LR (n = 3), HR (n = 3) and control unvaccinated (n = 3) pigs using Affymetrix microarray platform. *Immunological studies: cellular and humoral immune responses (serum, PBMCs). **Transcriptional studies (microarray analysis using the PBMCs) HR: high responders; LR: low responders; †The highest and the ††lowest average levels of virus-specific IFN-γ secreting cells through the time course of the study. Pre-vaccination (Pre-V). Post-vaccination (PV). Intramuscular (IM). Sterile Saline solution (SS).

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Veterinary Research

ISSN: 1297-9716