SAV RNA kinetics, early co-infection. SAV ΔCt values (SAV Ct–EF1α Ct) in blood and heart from individual co-challenged fish (black dots) and SAV control fish (red x) assayed by RTqPCR targeting SAV RNA segments. Red arrow indicates introduction of SAV2 shedders (A, B) or SAV3 shedders (C, D). Weeks post SAV shedder introduction (WPC-SAV) are indicated at the x-axis. N positive individuals (raw Ct < 37.0) of total sampled fish is tabulated under each sub-figure. Asterisk indicates significant (p < 0.05) differences between co-challenged groups and SAV control group.