Genetic, histochemical and biochemical studies on goat TSE cases from Cyprus

Susanne Niedermeyer; Martin Eiden; Pavlos Toumazos; Penelope Papasavva-Stylianou; Ioannis Ioannou; Theodoros Sklaviadis; Cynthia Panagiotidis; Jan Langeveld; Alex Bossers; Thorsten Kuczius; Martin Kaatz; Martin H. Groschup; Christine Fast

doi:10.1186/s13567-016-0379-0

Veterinary Research

Table 1 Data and results of scrapie positive Damascus goats from Cyprus

From: Genetic, histochemical and biochemical studies on goat TSE cases from Cyprus

Goat ZYP	Flock (age)	Brain stem	CM GC	Ln. retroph.	Tonsil	3^rd eye lid	Spleen	Rectum		Placenta
Goat ZYP	Flock (age)	IHC (rapid test)	CM GC	Ln. retroph.	Tonsil	3^rd eye lid	Spleen	Follicles	ENS	Placenta
I₁₄₂N₁₄₆R₁₅₁R₁₅₄R₂₁₁Q₂₂₂
3	B (4)	+++ (1.892)	+	NA	+++ 9/9	+++ 13/19	++ 83/202	+++ 1/1	neg.	neg.
8	C (4)	+++ (1.675)	NA	+++ 4/4	neg. 0/5	+++ 7/12	+ 6/140	neg. 0/3	+ Pl.my.	NA
9	D (4)	++ (1.579)	++	+++ 625/830	+++ 96/120	+++ 35/64	+++ 110/162	+++ 19/24	neg.	neg.
10	E (3)	+++ (1.985)	NA	+++ 232/646	++ 124/213	+++ 5/18	+ 93/428	+++ 37/48	+ Pl.my.	neg.
11	F (4)	+++ (1.856)	+	+ 93/284	+++ 124/255	neg. 0/18	+ 10/374	neg. 0/19	+ Pl.my.	neg.
12	G (3)	+++ (2.193)	++	++ 190/502	0	+ 9/46	+ 126/396	++ 71/152	neg.	neg.
13	G (4)	+++ (1.992)	+++	+ 87/365	+++ 111/145	+++ 4/4	+++ 56/99	+++ 131/174	++ Pl.my/+sub.	NA
14	F (4)	+++ (1.871)	+++	+++ 77/139	++ 174/307	+++ 5/5	+++ 167/246	+++ 28/46	++ Pl.my/+sub.	neg.
16	H (4)	+++ (2.204)	+++	+ 147/363	+ 20/78	+ 1/10	+ 64/270	++ 19/47	+ Pl.my/+sub.	NA
17	H (4)	+++ (2.107)	++	++ 119/300	+++ 72/132	+++ 15/17	+ 65/160	+++ 29/43	+ Pl.my.	NA
19	H (4)	++ (1.831)	+	++ 50/131	+ 15/82	+ 1/9	+++ 148/234	+++ 2/2	++ Pl.my.	NA
20	I (3)	+++ (1.961)	+	++ 18/57	+++ 268/335	+ 2/7	++ 123/373	+++ 26/43	++ Pl.my/+sub.	neg.
21	J (5)	++ (1.511)	+++	+ 190/615	NA	+ 4/41	++ 175/314	+ 20/37	+++ Pl.my/+sub.	+
22	J (5)	++ (1.151)	NA	NA	+++ 65/96	neg. 0/2	+ 42/269	+ 1/41	+ Pl.my.	NA
25	K (3)	++ (1.227)	++	+ 278/654	+++ 376/585	+++ 3/4	++ 173/339	++ 4/14	++ Pl.my/+sub.	NA
26	L (4)	++ (1.731)	+	+++ 196/302	0	neg. 0/26	+ 133/571	+ 55/146	+++ Pl.sub.	+
27	M (3)	++ (1.141)	+	+++ 229/344	+++ 41/65	+++ 17/23	+++ 82/152	++ 10/28	+++ Pl.my/+sub.	+
29	M (4)	++ (1.921)	++	+++ 180/313	+ 290/459	+++ 67/85	+++ 125/128	+++ 21/37	++ Pl.my/+sub.	neg.
30	M (6)	+++ (1.246)	++	++ 90/286	+++ 72/139	neg. 0/2	+ 81/224	+++ 17/22	+ Pl.my/+sub.	neg.
31	N (4)	++ (1.255)	++	+++ 264/398	+++ 131/144	+++ 26/55	++ 169/366	+++ 66/92	+++ Pl.my/+sub.	neg.
33	O (2)	++ (1.784)	++	+++ 136/262	+++ 141/164	++ 3/10	++ 257/503	+++ 30/39	++ Pl.my/+sub.	NA
34	P (2)	++ (1.376)	+++	+++ 626/878	+++ 191/256	+++ 8/9	+++ 228/292	+++ 6/7	+ Pl.my.	NA
35	P (4)	+++ (1.452)	NA	+++ 319/625	+++ 73/147	0	+ 4/208	+ 9/101	+ Pl.my.	neg.
41	O (4)	++ (1.674)	NA	++ 97/268	+++ 72/102	neg. 0/14	+ 60/167	0	+ Pl.my.	NA
24	K (2)	neg. (0.012)	+	+ 391/1154	NA	neg. 0/42	+ 11/446	+ 8/80	neg.	NA
37	Q (3)	neg. (0.010)	+	neg. 0/168	neg. 0/73	neg. 0/8	neg. 0/149	neg 0/14	neg.	NA
R/H154
40	R (4)	++ (1.069)	+	+ 21/220	neg. 0/6	neg. 0/6	neg. 0/134	neg. 0/35	+++ Pl.my.	neg.

D: aspartic acid; I: isoleucine; H: histidine; M: methionine; N: asparagine; Q: glutamine; R: arginine; S: serine; A–T: goat herds; age: is given in years; Cut-off BioRad rapid test: 0.213; IHC: immunohistochemistry; +, ++, +++: mild, moderate, severe PrP^Sc accumulation (the first number indicates the number of positive follicles, the second number the total number of follicles examined); CMGC: celiac and mesenteric ganglion complex; Ln. retroph.: retropharyngeal lymph node; 0: no lymphatic follicles; ENS: enteric nervous system; Pl. my.+sub.: myenteric/submucosal plexus; neg.: PrP^Sc negative; NA: tissue sample not available.

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