The activation of c-Jun following BHV-1 infection depended on JNK signaling. A Time course of altered phosphorylation of c-Jun at Ser73 following BHV-1 infection. Growth arrested MDBK cells were mock infected or infected with BHV-1 at an MOI of 10. At the indicated time points, the cells were lysed and subjected to Western blotting analysis using specific antibodies. B UV-irradiation inactivated virus still induced activation of c-Jun. MDBK cells were infected with BHV-1 (MOI = 10) or with equal amounts of UV-irradiation inactivated virus for 30 min. The cells were lysed for Western blotting. C, D C-Jun was activated by BHV-1 through JNK. MDBK cells were first pretreated with inhibitor SP600125, SB203580 and U0126 specific for JNK, p38MAPK and Erk1/2 signaling at the indicated concentrations for 1 h, and then infected with BHV-1 at an MOI of 10. At 0.5 and 24 hpi, the cells were respectively lysed for Western blotting to detect the phospho-c-Jun. Data are representative results of three independent experiments.