Table 1 Sequences of the primers used for real-time PCR and the siRNA against chGRP78
From: Membrane-associated GRP78 helps subgroup J avian leucosis virus enter cells
|  | Name | Sequence(5′–3′) |
|---|---|---|
RT-PCR | ALV-J gp37 | Forward: TGCGTGCGTGGTATTATTTC |
Reverse: AATGGTGAGGTCGCTGACTGT | ||
chGRP78 | Forward: GACGATGAGGAGAAAAAGGAG | |
Reverse: TGAATACACCCACACAAGAAT | ||
Chicken 18s | Forward: TCAGATACCGTCGTAGTTCC | |
Reverse: TTCCGTCAATTCCTTTAAGTT | ||
Human β-actin | Forward: CACGAAACTACCTTCAACTCC | |
Reverse: CATACTCCTGCTTGCTGATC | ||
siRNA | 366 | Forward: AGGACAUCAAGUAUCUGCCCUUCAA |
Reverse: UUGAAGGGCAGAUACUUGAUGUCCU | ||
611 | Forward: GGGUUGAACGUGAUGCGCAUUAUUA | |
Reverse: UAAUAAUGCGCAUCACGUUCAACCC | ||
519 | Forward: CCCACAGAUUGAAGUUACCUUUGAA | |
Reverse: UUCAAAGGUAACUUCAAUCUGUGGG |