siRNA against chGRP78 in DF1 cells. The cells were transfected with siRNA (50 pmol) against chGRP78 for 6 h, and the cells were then infected with ALV-J at an MOI of 1 for 72 h. A The siRNA effects on chGRP78 were detected with real-time PCR. B The infection/replication of ALV-J was analysed with Western blot. Lanes 1, 2, 3 and 4, DF1 cells transfected with siRNA 519, 611, 366 and Mix (co-transfected with 519, 611 and 366), respectively, against chGRP78. Lane 5, DF1 cells transfected with control siRNA. C TCID50 analysis of the viral titres.