Inhibition of ALV-J infection by antibodies to GRP78. The DF1 cells that had been pre-treated with antibodies against chGRP78 were infected with ALV-J, and ALV-J replication in the treated cells was analysed. A IFA analysis using JE9; B TCID50 analysis for viral titres; C Western blot analysis for the expression of env from ALV-J in the DF1 cells that had been treated with antibodies. Lane 1, blank; lane 2, mock; lane 3, goat IgG (50 μg/mL); lane 4, anti-GRP78 (5 μg/mL); lane 5, anti-GRP78 (25 μg/mL); lane 6, anti-GRP78 (50 μg/mL); D Western blot analysis of the expression of ALV-A p27 in the DF1 cells treated with antibodies. Lane 1, anti-GRP78 (50 μg/mL); lane 2, goat IgG (50 μg/mL); lane 3, mock.