Figure 4

Bacterial growth was affected by pyk deletion and recovered by supplementation with sodium pyruvate. A Growth curves in TSB. B Growth curves in minimal medium with or without sodium pyruvate. C Growth curves in minimal medium without glucose or replaced glucose by pyruvate as sole carbon source at concentration of 200 mM. Each point represents the mean for triplicate samples (error bars are within the size of the symbols). The experiment was repeated three times with similar results. *p ≤ 0.05; ***p ≤ 0.001.