Table 1 Primers used to quantify gene expression in mock or IFN-treated CEF using real-time qRT-PCR
Gene | Accession number | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|---|
GAPDH | NM_204305.1 | GGCACTGTCAAGGCTGAGAA | TGCATCTGCCCATTTGATGT |
IFNβ | NM_001024836.1 | CAGTCTCCAGGGATGCACAG | GAGAAGGTGGTGGTGAGAGC |
MX1 | NM_204609.1 | CACACCCAACTGTCAGCGAT | ATGTCCGAAACTCTCTGCGG |
IFIT5 | XM_421662.4 | TGCTTCACCAGCTAGGACTCTGC | TGGCTTTTGCTCTGTCACCACTTTG |
ZC3HAV1 | NM_001012938.1 | TCGGCGCCTCTCTACGCCAT | TCAGTCCACTGGCCGTGGTCA |
IRF8 | NM_205416.1 | ACAAGCAGGGCATCTTCATC | TGTTCCCACTCCAGAAGACC |
SOCS1 | NM_001137648.1 | CTGCTGGATGCCTGCGGCTT | GGGCCCGGTCGCGGTTTTAA |
IL15 | NM_204571.1 | CACTGTAAGTGGTCAGACGTTCTGA | GGTTCCTGGCATTCTATATCCTCGT |
RSAD2 | XM_426208.4 | GGACAAGGACGAGACAGTTCC | TCCCGCCTCCTTAAGCATTG |
TRIM25 | XM_415653.5 | TCAAGAGTCCCACCCTTCCA | AGCAGCTCAATGGACAGCAT |
LGP2 | HQ845773.1 | ATCTCGCGGCATTGTCTTCA | CTGCTGCTCATTCTGGGTCA |