Figure 2

Effect of orally fed BLS-mix on peripheral blood lymphocytes. Peripheral blood samples were collected from the indicated pigs at 0, 24, and 144 h after F4⁺ ETEC/VTEC/EPEC challenge. A FSC-A/SSC-A dot plot of peripheral blood lymphocytes, cells with no gating. B CD3 dot plot, cells were gated on lymphocytes. C CD4/CD8 dot plot, cells were gated on CD3⁺. Flow cytometry analysis of the percentages of D CD4⁺CD8⁻, E CD4⁻CD8⁺, F CD4⁺CD8⁺, G CD4⁻CD8⁻ cells among CD3⁺ T cells. Data are presented as the mean ± SEM for each time point (n = 8 per group). *P < 0.05; ***P < 0.001.