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Figure 2 | Veterinary Research

Figure 2

From: “Self-cleaving” 2A peptide from porcine teschovirus-1 mediates cleavage of dual fluorescent proteins in transgenic Eimeria tenella

Figure 2

“Self-cleaving” 2A peptide cleaves EYFP and RFP in EtER. A EtER sporozoites were reacted with mouse anti-SAG13 polyclonal antibody, followed by the reaction with AMCA-conjugated goat anti-mouse IgG (H + L) (Proteintech, USA), revealed by a confocal laser scanning microscopy (SP5, Leica, Germany). EYFP mainly localized to the nucleus, while RFP was found in the cytoplasm. Bar 5 μm. B RFP was secreted into PV in trophozoites (24 hpi) and 1st-genernation schizont stages, while EYFP in the nucleus (48 and 72 hpi). Bar 5 μm. C Soluble proteins extracted from EtER and the wild-type E. tenella (WT) were resolved by SDS-PAGE and the immunoblot analysis was conducted following standard protocols. The primary antibody was the mouse anti-EYFP polyclonal antibody and mouse anti-His tag monoclonal antibody, the mouse anti-GAPDH polyclonal antibody served as the loading control, while the HRP-conjugated goat anti-mouse IgG was used as the secondary antibody. TgDHFR-EYFP was 95 kDa and RFP 28 kDa. M: marker.

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Veterinary Research

ISSN: 1297-9716