Kinetics of the West Nile virus induced transcripts of selected cytokines and Toll-like receptors in equine peripheral blood mononuclear cells

Uddin, Muhammad Jasim; Suen, Willy W.; Bosco-Lauth, Angela; Hartwig, Airn-Elizabeth; Hall, Roy A.; Bowen, Richard A.; Bielefeldt-Ohmann, Helle

doi:10.1186/s13567-016-0347-8

Veterinary Research

Table 1 List of primers used in this study

From: Kinetics of the West Nile virus induced transcripts of selected cytokines and Toll-like receptors in equine peripheral blood mononuclear cells

Gene	Primer set^a	Amplicon size (bp)	Gene Bank accession number
ACTB	F: TGAGCGCAAGTACTCCGTAT R: TCCTGCTTGCTGATCCACAT	96	NM_001081838.1
GAPDH	F: GGTGAAGGTCGGAGTAAACG^b R: AATGAAGGGGTCATTGATGG	106	NM_001163856.1
IFNα	F: TCTTGATGCTCCTGGGACAA R: AACTGGTTGCCGTCAAACAC	103	NM_001099441.1
IFNβ	F: ACCATTCTGCGCCTGAAGAA R: AGGCCAAGTTCCTGAGCATT	115	NM_001099440.1
IFNγ	F: TTAACAGCAGCACCAGCAAG R: TTTGCGCTGGACCTTCAGAT	80	NM_001081949.1
TNFα	F: AAGCCTGTAGCCCATGTTGT R: TCTGTCAGCTTCACGCCATT	104	NM_001081819.1
IL1α	F: ATGAGGATCGTCAACCACCA R: TTCACTGCGTCGTCCAGATT	119	NM_001082500.1
IL1β	F: TCAAAGTCAGCCTGGTGGAA R: TTGCTCATCAGAAGCTGGGT	80	NM_001082526.1
IL6	F: TCTTCACAAGCACCGTCACT R: AGTGGTCCATTTGAGGTGGT	118	NM_001082496.1
IL8	F: TCAAGACGCACTCCAAACCT R: AGCTCCGTTGACGAGCTTTA	113	NM_001083951.1
IL12	F: ATCGTGGTGGATGCTGTTCA R: TAATGGCTTCAGCTGCAGGT	111	NM_001082516.1
IL22	F: TTCCAGCCTTACATGCAGGA R: TGCTGGTCATCACCCTCAAT	89	XM_001491754.3
PTX3	F: TCTGTGCAGCACCTGGAATT R: ACAACATGACCTGTGGCCAT	99	XM_005602088.1
CXCL10	F: GCACGCTGTACCTGCATTAA R: TGGCAATGATCTCAACACGT	112	NM_001114940.1
TLR1	F: TGTGGTGCCTTTAACAGCCT R: TTCTGGCAGCTCTGGAAGAA	89	NM_001256899.1
TLR2	F: TGCGAATCCTGAAAGTGGGA R: ACCGCTGGAGATTTGTAGCA	111	NM_001081796.1
TLR3	F: CCTGCAACAAGTGTGCTGTT R: TGGGTGAGATTCAACACCGT	109	NM_001081798.1
TLR4	F: AGCACTTCATTCAGAGCCGA R: TGAAGATGATGCCAGCACGA	90	NM_001099769.1
TLR5	F: TCCATGGAGGGTTGTGATGA^c R: CCCCGGAACTTTGTGACAAT
TLR6	F: CTGGCAAGAGCATTGTGGAA R: ATGGCACCACTCACTCTGAA	101	NM_001257142.1
TLR7	F: TTGAGTGGCCAAGAAACCCA R: ACCGTCTCTTTGAACACCTGA	109	NM_001081771.2
TLR8	F: TGGGCAAGTACGTGACAGAA R: GTGCCTTGCCATTGTGGTTT	120	NM_001111301.1
TLR9	F: AGCATCTTCGCACAAGACCT R: AGGTGGTGCAACATAGGCAT	116	NM_001081790.1
TLR10	F: TCAAACTCTCCTGCAGCCAA R: AGTTGTTTGACCGCCGAGAT	64	XM_005608830.1
MyD88	F: TGTGTTCCACTTGCCTCTCA R: ACGCAGGACATGAGATGTGA	97	XM_001488549.4
TRAF3	F: ACGACCAGATGCTGAGTGTT R: CCAAATCAGCACGCCATTGT	98	XM_001490000.3
STST1	F: TGCCTTGATCAGCTGCAGAA R: TGCTCCAGTTCCTCAAGCTT	92	XM_005601647.1
STST2	F: TGGAAAGTCCAGCAGCAGAA R: TGCTCCAGCTGTGAACCAAT	87	XM_001504841.2
IRF3	F: AAGGTTGTTCCCACATGCCT R: GTGGCTGTTGGAAATGTGCA	102	XM_005596407.1
IRF7	F: TGTTGTCACACTCATCGCCA R: ACGTGTGGATTGAGGGCTTA	80	XM_005598388.1
NFkB	F: ACCAGTGTCATCGAGCAGAT R: TGCAGGTGGTTGGTGAGATT	80	XM_001916418.3
ISG15	F: CAGTTCTGGCTGACTTTCGA R: CAGGCGCAAGTTCATGTACA	102	XM_005607605.1
SOCS1	F: ATTTAACTGTGTCTGGCGCC R: CAACCCCTGGTTTGTGCAAA	81	XM_005615004.1
SOCS3	F: TCTCCAACATCTCTGTCGGA R: TTAAAGCGGGGCATCGTACT	115	NM_001123379.1
Caspase 3	F: ATGCAGCAAACCTCAGGGAA R: ATGCCACAATTTCTTCGCCG	87	NM_001163961.1
HMOX1	F: TGGCTTCTTCCTTTGGGCAT R: TGCTTGTTGGTTGGGGAAGA	108	XM_005606579.1
WNV_NSW2011	F: AACCCCAGTGGAGAAGTGGA^d R: TCAGGCTGCCACACCAAA	70	D00246

^aAnnealing temperature was 60 °C for all the primer sets.
^bPrimer set is adopted from Beckman et al. [54].
^cKwon S et al. [55].
^dPyke et al. [35].

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ISSN: 1297-9716

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