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Figure 8 | Veterinary Research

Figure 8

From: Kinetics of the West Nile virus induced transcripts of selected cytokines and Toll-like receptors in equine peripheral blood mononuclear cells

Figure 8

Growth kinetics of WNV NSW2011 and detection of viral antigen in equine PBMCs in vitro. A Viral RNA quantification in PBMCs using qRT-PCR, (B) Virus replication in cells quantified using plaque assay. *PC: positive control, NC: negative control. Thermal inactivated curve is superimposed by the other lines. C WNV-stimulated PBMCs immunostained using flavivirus NS1 specific monoclonal antibody 4G4, (D) WNV-stimulated PBMCs immunostained without 4G4, (E) Mock-stimulated PBMCs immunostained using 4G4 as negative control, and (F) WNV-stimulated Vero immunostained using 4G4 as positive control. (Magnification: ×20). Arrow, indicated the perivascular regions.

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Veterinary Research

ISSN: 1297-9716