Table 1 Primer sequences for real-time PCR analyses of cytokine transcript expression
Primer | F/R | Nucleotide sequencing (5′-3′) | Accession no |
|---|---|---|---|
GAPDH | Forward | TGC TGC CCA GAA CAT CAT CC | NM_204305 |
Reverse | ACG GCA GGT CAG GTC AAC AA | ||
CCL3 | Forward | CAT TGC CTC CGC CTA CAT | EU999777 |
Reverse | ACT CCT CGG GGT TTA CAC ATA | ||
CCL4 | Forward | CCC CTT GTC ATC GGT CAC | NM_204720 |
Reverse | AGA GGC AGG AGC AGA GCA | ||
CCL5 | Forward | CAG CAA ATG CCC ACA GG | NM_001045832 |
Reverse | TGC AGC TCC AGG AAG TTG AT | ||
CCL20 | Forward | AGG CAG CGA AGG AGC AC | NM_204438 |
Reverse | GCA GAG AAG CCA AAA TCA AAC | ||
CXCL13 | Forward | GCC TGT GCC TGG TGC TC | XM_420474.4 |
Reverse | TGC CCC CTT CCC CTA AC | ||
IFN-γ | Forward | AGC TGA CGA CGG TGG ACC TAT TAT T | HQ739082 |
Reverse | GGC TTT GCG CTG GAT TC | ||
IL-4 | Forward | AGC ACT GCC ACA AGA ACC TG | NM_001007079 |
Reverse | CCT GCT GCC GTG GGA CAT | ||
IL-6 | Forward | CAA GGT GAC GGA GGA GGA C | JQ897539 |
Reverse | TGG CGA GGA GGG ATT TCT | ||
IL-8 (CXCLi2) | Forward | GGC TTG CTA GGG GAA ATG A | NM_205498 |
Reverse | AGC TGA CTC TGA CTA GGA AAC TGC | ||
IL-10 | Forward | CTG TCA CCG CTT CTT CAC CT | AJ621254 |
Reverse | ACT CCC CCA TGG CTT TGT A | ||
IL-16 | Forward | TGC CTC ACA AGA ATC AAC AAC T | AB104417.2 |
Reverse | ATA GAG CCC TTC CCA CCT TC | ||
IL-17A | Forward | TGT CTC CGA TCC CTT GTT CT | AM773756 |
Reverse | GTC CTG GCC GTA TCA CCT T | ||
IL-17D | Forward | ACC CCA CAA GAT ACC CTA AAT AC | EF570583 |
Reverse | GTG CTG CGG AAG TGA AAA T | ||
IL-17F | Forward | CTC CGA TCC CTT ATT CTC CTC | NM_204460 |
Reverse | GTC CTG GCC GTA TCA CCT T | ||
IL-18 | Forward | ATT TTC CCA TGC TCT TTC TCA | HM854281 |
Reverse | GGA ATG CGA TGC CTT TTG | ||
IL-26 | Forward | AAT GCC TGT CTT CCG TGT G | XM_004937561 |
Reverse | TCA TTG ATG GCC TTG TAG ACC | ||
TNFSF15 | Forward | CCT GAG TAT TCC AGC AAC GCA | NM_001024578 |
Reverse | ATC CAC CAG CTT GAT GTC ATC AAC | ||
IL-10R2 | Forward | GAG CAG ACC ACC CAT AAC G | NM_204857 |
Reverse | ATA CCA AAA GGC AAA GAA ACA A | ||
IL-20R1 | Forward | GTG GAT GAA TTG CTG GGT AAG | XM_419723 |
Reverse | TCT GTG ATG CCG TGT GCT AT |