Effect of BHV-1 on mitochondrial membrane potential in MDBK cells. (A) MDBK cells of confluent in 96-well plates were infected with BHV-1 at MOI of 0.01 for indicated time length, and then MMP was detected with JC-1 dye. The treatment of cells with CCCP for 20 min was used as a positive control. (B) MDBK cells of confluent in 96-well plates were treated with NAC and GSH at indicated concentrations, before and during virus infection with BHV-1 (MOI = 0.01) for 48 h. The MMP was detected with JC-1 dye. Data represent three independent experiments. Statistical analyses were performed using Student’s t test (Asterisk indicates P < 0.05 vs. mock).