Skip to main content

Table 3 Infection titers of a WSSV stock in P. vannamei without and with removal of the peritrophic membrane.

From: Per os infectivity of white spot syndrome virus (WSSV) in white-legged shrimp (Litopenaeus vannamei) and role of peritrophic membrane

Experiment Inoculation route Removal of PM Dilution tissue homogenate No. of shrimp Mortality at different time points (hpi) Infection by IIF Virus titer
24 36 48 60 72 84 96 120 Total
1 Intramuscular No 10−6 5   2 2 1      5/5 5/5 108.5 SID50/mL
No 10−7 5    1 2      3/5 3/5  
No 10−8 5          0/5 0/5  
Peroral No 100 5    2       2/5 2/5 101.1 SID50/mL
No 10−1 5          0/5 0/5  
No 10−2 5          0/5 0/5  
No 10−3 5          0/5 0/5  
Yes 100 5   1 1       2/5 2/5 101.3 SID50/mL
Yes 10−1 5       1    1/5 1/5  
Yes 10−2 5          0/5 0/5  
Yes 10−3 5          0/5 0/5  
2 Intramuscular No 10−6 5   2 1 2      5/5 5/5 108.7 SID50/mL
No 10−7 5    2 2      4/5 4/5  
No 10−8 5          0/5 0/5  
Peroral No 100 5   1        1/5 1/5 101.2 SID50/mL
No 10−1 5     1      1/5 1/5  
No 10−2 5     1      1/5 1/5  
No 10−3 5          0/5 0/5  
Yes 100 5    1 2      3/5 3/5 101.6 SID50/mL
Yes 10−1 5    1       1/5 1/5  
Yes 10−2 5          0/5 0/5  
Yes 10−3 5          0/5 0/5  
3 Intramuscular No 10−6 15   2 4 4 4 1    15/15 15/15 108.7 SID50/mL
No 10−7 15   1 2 4 4 1    12/15 12/15  
No 10−8 15          0/15 1/15  
Peroral No 100 15   1 1 3      5/15 5/15 101.1 SID50/mL
No 10−1 15   1        1/15 1/15  
No 10−2 15          0/15 0/15  
No 10−3 15          0/15 0/15  
Yes 100 15   2 3 2 3 1    11/15 11/15 101.7 SID50/mL
Yes 10−1 15    2       2/15 2/15  
Yes 10−2 15          0/15 0/15  
Yes 10−3 15          0/15 0/15