Viral RNA loads in the nervous system following experimental infection of pigs with JEV. At necropsy nervous tissues were sampled and homogenized before quantitative RT-PCR was run. Relative RNA quantities are shown for each day (A–D) calculated in U/mL. Bars indicate mean values of a group. To illustrate RT-PCR negative samples they were plotted as 10−1 U/mL. *difference statistically significant (p < 0.05) when this organ is compared to all others for a particular time point pi.