Figure 4

Characterization of VRP-mediated expression of N. A Immunofluorescence analysis of MARC-145 cells 6 h after infection with VSV*ΔG(N) or with the VSV*ΔG control. The expression of N is detected with the anti-N mAb 13E2. B Western blot analysis of lysates from non-infected cells (lane 1) or cells infected with VSV*ΔG (lane 2), VSVΔ*G(N) (lane 3), PRRSV Olot/91 (lane 4) or mock (lane 5). The proteins were separated by SDS-PAGE under non-reducing (-βME) conditions and blotted onto a nitrocellulose membrane. N antigen was detected with the anti-N mAb as above. Protein molecular weight (kDa) standards are indicated.