Production of P25 protein in cell culture. Western Blot of ovine fibroblasts (OSF) infected with supernatants from red deer (DSF), fallow deer (FSF) and OSF infected with strains Ev1 (A) or 496 (B) after 7 days of infection. Uninfected cells and recombinant p25 protein were also included as negative and positive controls respectively. Sera from two experimentally infected sheep with SRLV strains from genotype A and B respectively, were used to reveal protein production. Beta tubulin antibody was used as loading control.