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Table 2 Extent and kinetics of modulated mRNA concentrations after stimulating pbMEC with native LTA from S. uberis strain 233 and T1–18.

From: Streptococcus uberis strains isolated from the bovine mammary gland evade immune recognition by mammary epithelial cells, but not of macrophages

Gene LTA from strain Concentration (µg/mL) Time
1 h 3 h 24 h
TNF 233 1 4.6 ± 0.3 68 ± 1.8 3.2 ± 0.2
10 5.7 ± 0.0 110 ± 2.9 14.1 ± 0.2
T1–18 1 3.6 ± 0.3 44 ± 10.0 2.8 ± 0.1
10 6.1 ± 0.3 114 ± 2.2 13.4 ± 0.3
IL6 233 1 1.2 ± 0.0 10.2 ± 0.8 1.9 ± 0.1
10 1.3 ± 0.1 18.5 ± 0.2 4.3 ± 0.04
T1–18 1 1.2 ± 0.1 7.1 ± 1.7 1.7 ± 0.1
10 1.2 ± 0.1 18.5 ± 0.6 4.1 ± 0.1
CXCL8 233 1 2.9 ± 0.05 38 ± 2.7 3.2 ± 0.0
10 3.2 ± 0.1 69 ± 1.1 10.2 ± 0.2
T1–18 1 2.1 ± 0.1 25 ± 4.3 2.5 ± 0.3
10 3.2 ± 0.2 74 ± 1.7 9.3 ± 0.1
CCL5 233 1 0.9 ± 0.01 6.6 ± 0.3 6.0 ± 0.1
10 1.1 ± 0.1 14.7 ± 0.6 28 ± 1.1
T1–18 1 1.0 ± 0.01 3.7 ± 1.2 4.9 ± 0.4
10 1.0 ± 0.1 13.4 ± 1.2 33 ± 2.8
NOS2A 233 1 1.0 ± 0.04 55 ± 3.2 2.0 ± 0.1
10 1.0 ± 0.02 119 ± 5.4 7.3 ± 0.1
T1–18 1 0.9 ± 0.13 31 ± 12.8 1.7 ± 0.1
10 1.0 ± 0.05 112 ± 9.7 6.1 ± 0.3
LAP 233 1 1.0 ± 0.08 2.9 ± 0.08 17 ± 3.5
10 1.0 ± 0.04 4.6 ± 0.48 47 ± 1.0
T1–18 1 0.9 ± 0.05 2.2 ± 0.22 11 ± 1.4
10 1.0 ± 0.04 4.2 ± 0.03 39 ± 0.7
SAA3 233 1 1.1 ± 0.02 34 ± 4.6 31 ± 2.2
10 1.1 ± 0.00 50 ± 0.6 124 ± 30
T1–18 1 1.1 ± 0.02 23 ± 6.9 45 ± 8.0
10 1.2 ± 0.01 50 ± 1.6 119 ± 18
CYP1A1 233 1 1.0 ± 0.02 1.7 ± 0.1 1.0 ± 0.04
10 2.1 ± 0.03 25 ± 1.5 1.5 ± 0.1
T1–18 1 1.0 ± 0.03 1.4 ± 0.2 1.3 ± 0.2
10 1.8 ± 0.11 20 ± 0.6 1.8 ± 0.1
  1. pbMEC were stimulated with 1 or 10 µg/mL LTA for the indicated time. Values are means (±SEM) of fold change relative to unstimulated control from two biological replica experiments, each assayed in duplicate; bold numbers represent significant regulation