Table 2 Extent and kinetics of modulated mRNA concentrations after stimulating pbMEC with native LTA from S. uberis strain 233 and T1–18.
Gene | LTA from strain | Concentration (µg/mL) | Time | ||
|---|---|---|---|---|---|
1Â h | 3Â h | 24Â h | |||
TNF | 233 | 1 | 4.6 ± 0.3 | 68 ± 1.8 | 3.2 ± 0.2 |
10 | 5.7 ± 0.0 | 110 ± 2.9 | 14.1 ± 0.2 | ||
T1–18 | 1 | 3.6 ± 0.3 | 44 ± 10.0 | 2.8 ± 0.1 | |
10 | 6.1 ± 0.3 | 114 ± 2.2 | 13.4 ± 0.3 | ||
IL6 | 233 | 1 | 1.2 ± 0.0 | 10.2 ± 0.8 | 1.9 ± 0.1 |
10 | 1.3 ± 0.1 | 18.5 ± 0.2 | 4.3 ± 0.04 | ||
T1–18 | 1 | 1.2 ± 0.1 | 7.1 ± 1.7 | 1.7 ± 0.1 | |
10 | 1.2 ± 0.1 | 18.5 ± 0.6 | 4.1 ± 0.1 | ||
CXCL8 | 233 | 1 | 2.9 ± 0.05 | 38 ± 2.7 | 3.2 ± 0.0 |
10 | 3.2 ± 0.1 | 69 ± 1.1 | 10.2 ± 0.2 | ||
T1–18 | 1 | 2.1 ± 0.1 | 25 ± 4.3 | 2.5 ± 0.3 | |
10 | 3.2 ± 0.2 | 74 ± 1.7 | 9.3 ± 0.1 | ||
CCL5 | 233 | 1 | 0.9 ± 0.01 | 6.6 ± 0.3 | 6.0 ± 0.1 |
10 | 1.1 ± 0.1 | 14.7 ± 0.6 | 28 ± 1.1 | ||
T1–18 | 1 | 1.0 ± 0.01 | 3.7 ± 1.2 | 4.9 ± 0.4 | |
10 | 1.0 ± 0.1 | 13.4 ± 1.2 | 33 ± 2.8 | ||
NOS2A | 233 | 1 | 1.0 ± 0.04 | 55 ± 3.2 | 2.0 ± 0.1 |
10 | 1.0 ± 0.02 | 119 ± 5.4 | 7.3 ± 0.1 | ||
T1–18 | 1 | 0.9 ± 0.13 | 31 ± 12.8 | 1.7 ± 0.1 | |
10 | 1.0 ± 0.05 | 112 ± 9.7 | 6.1 ± 0.3 | ||
LAP | 233 | 1 | 1.0 ± 0.08 | 2.9 ± 0.08 | 17 ± 3.5 |
10 | 1.0 ± 0.04 | 4.6 ± 0.48 | 47 ± 1.0 | ||
T1–18 | 1 | 0.9 ± 0.05 | 2.2 ± 0.22 | 11 ± 1.4 | |
10 | 1.0 ± 0.04 | 4.2 ± 0.03 | 39 ± 0.7 | ||
SAA3 | 233 | 1 | 1.1 ± 0.02 | 34 ± 4.6 | 31 ± 2.2 |
10 | 1.1 ± 0.00 | 50 ± 0.6 | 124 ± 30 | ||
T1–18 | 1 | 1.1 ± 0.02 | 23 ± 6.9 | 45 ± 8.0 | |
10 | 1.2 ± 0.01 | 50 ± 1.6 | 119 ± 18 | ||
CYP1A1 | 233 | 1 | 1.0 ± 0.02 | 1.7 ± 0.1 | 1.0 ± 0.04 |
10 | 2.1 ± 0.03 | 25 ± 1.5 | 1.5 ± 0.1 | ||
T1–18 | 1 | 1.0 ± 0.03 | 1.4 ± 0.2 | 1.3 ± 0.2 | |
10 | 1.8 ± 0.11 | 20 ± 0.6 | 1.8 ± 0.1 | ||