strains from clinical or subclinical cases of mastitis did not activate immune functions of pbMEC. Induction of gene expression (ordinate) of TNF, CXCL8 and CYP1A1 after challenging for 0, 1, 3 and 24 h (abscissa) with 30 µg/mL of 14 different heat-killed S. uberis strains isolated from clinical (A) or subclinical (B) cases of mastitis compared against E. coli
1303. Values are RT-qPCR measurements of the respective mRNA species, normalized against CLIC1 and expressed as multiple of the value measured from the unstimulated control. Values are from a single experiment, assayed in duplicate. Only E. coli
1303 elicited a significant induction (compared to control, p < 0.05) of TNF and CXCL8. All preparations strongly induced the expression of CYP1A1.