From: Roles of nucleic acid substrates and cofactors in the vhs protein activity of pseudorabies virus
Residues corresponding to PrV vhs | Corresponding residues | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
HSV vhs | T4 RNase H | Human FEN-1 | ||||||||
RNase activity [13] | vhs activity [24] | eIF4H binding [13] | Nuclease /substrate binding [33] | |||||||
D34 | D34 | − | − | + | D19 | − | − | D34 | − | − |
D81 | D82 | − | − | + | D71 | − | − | D86 | − | − |
K95 | K96 | − | − | N | K87 | N | − | R103 | N | + |
E150 | E192 | − | − | + | E130 | N | N | E158 | − | − |
D152 | D194 | − | − | + | D132 | − | − | E160 | − | N |
D169 | T211 | − | < | − | S153 | − | < | T177 | N | N |
D171 | D213 | − | − | + | D155 | − | − | D179 | − | + |
D172 | T214 | − | − | − | D156 | N | N | D180 | N | N |
D173 | D215 | − | − | + | D157 | − | ≪ | D181 | − | ∓ |
D219 | D261 | − | < | + | D200 | − | + | D233 | − | − |
P343 | P465 | N | N | − | − | N | N | E357 | N | N |
P345 | P467 | N | N | − | − | N | N | E359 | N | N |
L352 | L474 | N | N | − | − | N | N | K366 | N | N |
W356 | W478 | N | N | − | − | N | N | G370 | N | N |