Figure 3
Spleen and liver features and anti-
Leishmania
humoral response of infected hamsters. A Weights of spleen and liver of animals infected with JPCM5 strain and isolates from the focus were compared with organ weights from uninfected hamsters according to the route of infection. Bars represent the standard deviation. P < 0.05 was considered statistically significant. Spleen of hamster infected by sand fly bites with POL2FL6 showing severe splenomegaly (B) compared to spleen of non-inoculated animal (C). D Giemsa-stained imprint of spleen of hamster infected by sand fly bites with POL2FL6 showing macrophages packed with amastigotes. E Anti-L. infantum IgG antibody levels in sera of hamsters. ○: Control animals; □: Hamster group H1; 
: Hamster group H2; 
: Hamster group H3; 
: Hamster group H4; 
: Hamster group H5; 
: Hamster group H6; 
: Hamster group H7; 
: Hamster group H8; 0.59 OD cut off value was established with sera of non-infected hamsters.