Table 1 Primer sequences (cattle) and abbreviations of the genes used in the qRT-PCR assays, including GeneBank accession numbers.
Accession number | Primer sequence | |
|---|---|---|
Housekeeping genes | ||
GAPDH (glyceraldehyde-3-phosphate dehydrogenase) | NM_001034034.1 | F: ACCCAGAAGACTGTGGATGG R: CAACAGACACGTTGGGAGTG |
HPRT1 (hypoxanthine phosphoribosyltransferase 1) | NM_001034035.1 | F: CACTGGGAAGACAATGCAGA R: ACACTTCGAGGGGTCCTTTT |
RPS29 (ribosomal protein S29) | BC102702 | F: GGAGCCATCCGAGAAAATTCG R: CAACTTAATGAAGCCGATGTCCTT |
RLP0 (ribosomal protein P0) | NM_001012682.1 | F: CTTCATTGTGGGAGCAGACA R: GGCAACAGTTTCTCCAGAGC |
HDAC10 (histone deacetylase 10) | NM_001075460.1 | F: CCGATGACGGGAGAAATCTA R: CTCAGGAACCCACCAGTTGT |
SDHA (succinate dehydrogenase complex subunit A) | NM_174178.2 | F: ACATGCAGAAGTCGATGCAG R: GGTCTCCACCAGGTCAGTGT |
qRT-PCR PBMC and skin | ||
IL-2 (IL–interleukin) | NM_180997.1 | F: TCCAAGCAAAAACCTGAACC R: CAGCGTTTACTGTTGCATCATC |
IL-4 | NM_173921.2 | F: GCGGACTTGACAGGAATCTC R: TCAGCGTACTTGTGCTCGTC |
IL-5 | NM_173922.1 | F: TGGTGGCAGAGACCTTGACA R: TTCCCATCACCTATCAGCAGAGT |
IL-6 | NM_173923.2 | F: TCCTTGCTGCTTTCACACTC R: CACCCCAGGCAGACTACTTC |
IL-10 | NM_174088.1 | F: TGTATCCACTTGCCAACCAG R: CAGCAGAGACTGGGTCAACA |
IL-13 | NM_174089.1 | F: GGTGGCCTCACCTCCCCAAG R: ATGACACTGCAGTTGGAGATGCTG |
IL-17 | NM_001008412.1 | F: GGACTCTCCACCGCAATGAG R: TGGCCTCCCAGATCACAGA |
IL-23A | NM_001205688.1 | F: CCCGTATCCAGTGTGAGGAT R: AGTATGGAGGCGTGAAGCTG |
FOXP3 (forkhead box P3) | NM_001045933.1 | F: GACAGCACCCTTTCGACTGT R: CTCCAGAGATTGCACCACCT |
IFN-γ (interferon-γ) | NM_174086.1 | F: TTCTTGAATGGCAGCTCTGA R: TTCTCTTCGGCTTTCTGAGG |
NCR1 (natural cytotoxicity triggering receptor1) | NM_183365.1 | F: CTGAGAGCGTGGGTGTATCA R: CTGAGAGCGTGGGTGTATCA |
TGF-β1 (transforming growth factor β1) | NM_001166068.1 | F: CTGCTGTGTTCGTCAGCTCT R: TCCAGGCTCCAGATGTAAGG |
qRT-PCR skin | ||
AREG (amphiregulin) | BC141281.1 | F: TGGTCA R: GTCGATCACGGAGGACAGTT |
CCR3 (chemokine receptor 3) | NM_001194960.1 | F: TGTGTCAACCCCGTGATCTA R: AGAGTTCCTGCTCCCCTGTT |
FCERA1 (Fc IgE receptor α) | NM_001100310.1 | F: CAGAGGCTGCCCTACATCTC R: GTTTAGGCTGTGGGTCCGTA |
FIL (filaggrin) | * | F: GCCCAGTTCTAGACGCTGAC R: TCAAGCCAGTGACAGTGAGG |
IVL (involucrin) | * | F: AAGGTCTTGGGCCAGCACTTG R: GATGCTGGGTTGTAACTCCCCCCAC |
LOR (loricrin) | * | F: CAGTGGATCCGTCTGCCTGGGA R: CATGAGAGCGGTAAGCCCATCGAC |