Effect of free
galactan on secreted embryonic alkaline phosphatase (SEAP) release from HEK-Blue mTLR2 cells (
) and HEK-Blue mTLR4 cells (
). Null-cells (i.e., HEK-Blue cells neither expressing TLR2 nor TLR4) or TLR-cells were incubated with galactan (EPS) at 0.1, 1 and 10 µg/mL, or with FSL1 lipopeptide and E. coli LPS as positive controls for TLR2 and TLR4 cells respectively. SEAP release after stimulation was measured by a colorimetric method and expressed as mean fold changes (±SD) in comparison to unstimulated cells (n = 3). *p < 0.001 relative to unstimulated controls; **p < 0.05 between different EPS concentrations.