Figure 1
From: Keratin and S100 calcium-binding proteins are major constituents of the bovine teat canal lining
2-DE proteome map of bovine teat canal lining and teat skin. (A) Pooled TCL proteins (350 μg) were subjected to 2-DE and detected by colloidal Coomassie blue staining. The relative molecular weight (MW) is given on the left, while the pI range is given at the top of the figure. Thirty six protein spots corresponding to identified keratin proteins and 17 S100 protein spots (enclosed in the box) are indicated by arrows. Keratin abbreviations are (K1) keratin, type II, cytoskeletal 1; (K3) keratin, type II, cytoskeletal 3; (K4) keratin, type II, cytoskeletal 4; (K5) keratin, type II, cytoskeletal 5; (K6a) keratin, type II, cytoskeletal 6A; (K6c) keratin, type II, cytoskeletal 6C; (K10) keratin, type I, cytoskeletal 10; (K14) keratin, type I, cytoskeletal 14; (K17) keratin, type I, cytoskeletal 17; (K59) keratin, type II, cytoskeletal 59, component IV; (K79) keratin, type II, cytoskeletal 79. (B) Pooled teat skin proteins (350 μg) were subjected to 2-DE and detected by colloidal Coomassie blue staining. The absence or lowered relative abundance of keratin proteins, compared to TCL, is indicated by dotted circles. Keratin and S100 protein spots identified by MS are indicated by arrows. TCL, teat canal lining; TS, teat skin.