A combinational approach of multilocus sequence typing and other molecular typing methods in unravelling the epidemiology of Erysipelothrix rhusiopathiae strains from poultry and mammals

Janßen, Traute; Voss, Matthias; Kühl, Michael; Semmler, Torsten; Philipp, Hans-Christian; Ewers, Christa

doi:10.1186/s13567-015-0216-x

Veterinary Research

Table 2 Oligonucleotide primers used for multiplex PCRs to detect 16 genes putatively linked with virulence of E. rhusiopathiae

From: A combinational approach of multilocus sequence typing and other molecular typing methods in unravelling the epidemiology of Erysipelothrix rhusiopathiae strains from poultry and mammals

Gene / locus tag	Gene product / predicted function [reference]	Primer sequence (5′ – 3′) (forward/reverse)^a	GenBank Acc. No	Localization within gene	Size (bp)	Gene pre-valence (%)
ERH_1356	ABC transporter metal-binding protein / adhesion of host cells [45]	CATGAAGGGTAACACCTTGG/ GGGCGATAAAGTTGCGGTAGAA	NC_015601.1	579 – 787	209	100
intI-like	Internalin / invasion of epithelial cells [18,45]	ACAGTTTCGGATACTTCCGG/ ACCCTCGTCATATTTACCAGC	AP012027.1	386 – 714	329	85.5
rspB	Rhusiopathiae surface protein /biofilm formation [41]	ATCTTTACCCAATTCGACGT/ ATGAACCCAGTCCAAGATTGG	AB052682.1	6882 – 7287	406	100
rspA	Rhusiopathiae surface protein /biofilm formation [41]	ATCGACTGGTATTCAGTTGG/ ATCACGAGACATACCGCCAA	AB052682.1	597 – 1131	535	100
cap locus	Capsule / resistance to phagocytosis, intracellular survival [40]	TATCTTTGTAGCGTAGTTGG/ CAATAAAAGGAAATACCAGTGC	D64177	1333 – 1987	645	100
algI	Alginate-O-acetyltransferase / resistance to phagocytosis [45]	AGTTATCTTGGACTTGGTCC/ AGATAAGTGCGCATTGATCC	AP012027.1	121 – 887	767	97.6
ewlA	Lipoprotein / adhesion to host cells [45]	TAATATTAGATAGCGAGGAAT/ AAGAAAAGGGAGTGTGAATAT	U52850.1	226 –1185	960	100
nanH	Neuraminidase / spreading factor, nutritive [44]	ATGAAGCGCTTACATTTGAAT/ TACATAAGGTTGACCAAAGTC	AB019122	295 –1401	1.107	100
sub	S8-subtilisin / peptidase [45]	AAGCCTGAGATATCTGCACC/ TTGTACAATTGGATGAGCCG	AP012027.1	1360 –1585	226	100
sodA	Superoxide dismutase / antioxidans [45,70]	AGAAGACATCCGCACAGCAGT/ GCATGTTCCCAAACATCAAGA	AP012027.1	195 – 509	315	100
mviN1	Integral membrane protein / cell adhesion, transport protein [45]	AAATCATGCTTGTAATGGCGG/ ATTCGACGTTAAAACAACCGC	AP012027.1	565 –1032	378	100
hep	Heparinase / inactivation of heparin [45]	ATGGAAGTACCGATCTCACT/ TCATTGTAGCAACATGGCTTC	AP012027.1	997 –1480	484	100
hlyIII	Hemolysin / lytic activity on red blood cells [42]	TACGATTGCGACAAAGTGTGCG/ ATGGAAACATAGGGAAGGCTG	AP012027.1	16 – 559	544	100
fbpA	Fibronectin-binding protein / adhesion [41]	ATCTCGCCGCTTTTAGAACG/ GCGTCTTCAACTGTTGCTTG	AP012027.1	565 –1166	602	100
hlyA	Hyaluronidase / spreading factor [2]	AGGATCACTTACCGCTATGG/ CAGCACTCAGCATGTTCTC	AP012027.1	598 –1538	941	100
dnaB	Membrane-, attachment protein / proliferation, adhesion [45]	AATAGCCCCTGATCAAATGG/ CTCTCCTTTACTTAACATCCC	ACLK02000002.1	39 –1155	1.117	99.4

Primers were designed in this study except for the primers to amplify the cap locus [46].
^aIn case Ogawa et al. [45] is given as a reference, the predicted function of the gene product has been deduced from genome annotation data of strain Fujisawa. Here, in vitro or in vivo studies would be mandatory to verify the linkage of the genes with the pathogenesis of Erysipelas in different hosts.

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ISSN: 1297-9716

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