Figure 1

Purification of TAdV-3 virions. A Strategy for enrichment and purification of TAdV-3 virions. Flow diagram depicts the strategy. B CsCl2 purification of TAdV-3. The lower band containing mature viruses (left panel) subjected to second round of CsCl (right panel). C–E Analysis of TAdV-3 virions purity. Electron micrograph of CsCl purified TAdV-3 negatively stained with 2% aqueous phosphotungstic acid (Direct magnification 100000X) (C) and (direct magnification 150000X) (D). E Analysis of TAdV-3 polypeptides. Purified TAdV-3 proteins were separated by 10–15% SDS-PAGE and analyzed by Western blot using turkey anti-TAdV-3 serum [4,9] and peroxidase-linked goat anti-turkey IgG antibodies (right panel). The identified polypeptides (lane 1) are depicted. Molecular weight markers (MW) in kDa are shown on the left of the panel.