rbCyPA binds to granulocytes, monocytes and lymphocytes present in bovine peripheral blood. A A gating strategy for flow cytometry analysis was established by staining with anti-bovine granulocytes, CD14 or CD3. Three major populations (gate 1, 2 and 3) were found on the FSC and SSC profiles of bovine peripheral blood cells (1 left panel). Cells present in gate 1, 2 and 3 were granulocytes, monocytes and lymphocytes, respectively, because these were granulocyte+CD14−CD3− cells, granulocytelowCD14+CD3− cells and granulocyte−CD14+CD3+ or – cells (9 right panels), respectively. B rbCyPA reacted to granulocytes, monocytes and lymphocytes in a time-dependent manner. Longitudinal change is shown in the line graph on the right. Three separate experiments were performed and the data represent means ± SEM. Values with different letters are statistically different (p < 0.01).