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Table 4 Detection of T. gondii DNA from bioassay inocula (homogenised tissues) compared to detection of T. gondii DNA from mouse brain (bioassay) from oocyst challenged pigs (G1)

From: Vaccination of pigs with the S48 strain of Toxoplasma gondii – safer meat for human consumption

Pig No.

Brain

Other

Food

qPCR

ITS1

Bio

qPCR

ITS1

Bio

qPCR

ITS1

Bio

825

+

+

+

826

+

+

+

+

+

827

+

+

+

+

828

+

+

+

+

+

829

+

+

+

+

+

Positives

4/5

4/5

4/5

1/5

2/5

4/5

1/5

1/5

1/5

  1. Results from the 529 bp T. gondii qPCR (qPCR), conventional T. gondii ITS1 PCR (ITS1) and ITS1 PCR from mouse brain – bioassay result (Bio), from oocyst challenged pigs (G1).