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Table 1 sPMCA analysis of collected dust samples

From: Circulation of prions within dust on a scrapie affected farm

Dust collection area Number positive reactions
Control barn (10 dust sample extracts) 0/56
Equipment barn area 1 extract 1 0/5
        area 1 extract 2 0/5
Hay barn area 1 extract 1 0/5
    area 1 extract 2 0/5
Sheep barn 1 area 3 3/5
Sheep barn 1 area 4 1/5
Sheep barn 1 area 5 3/5
Sheep barn 2 area 11 2/5
Sheep barn 2 area 12 3/5
Polytunnel (30 m) extract 1 3/5
        extract 2 3/5
Polytunnel (60 m) extract 1 0/5
        extract 2 0/5
  1. Dust samples collected in petri-dishes were extracted and then subject to sPMCA. Detailed are the individual samples that were extracted and subject to 9 rounds of sPMCA before PK digestion and analysis by dot blot. sPMCA products were scored positive/negative by dot blot after round 9.