Efficiency of HA gene insertion in pBAC-C-KCE base on MAGIC and characterization of the recombinant virus C-KCE-HA.
(A) The genome BAC-C-KCE and C-KCE were isolated and digested with BamH I and separated with a 0.8% agarose gel. The red arrowhead shows that the band in line 3 are bigger than the band in line 4 (that is 8.3 kb by analyzing the genome using DNAman tool). The sizes of a molecular weight marker (15 000-bp and 5000-bp marker, Transgen) are given. (B) Detection of the HA gene insertion in pBAC-C-KCE by PCR. The marker used was DL15000. (C) Detection the expression of HA protein in C-KCE-HA-infected CEF by Western blotting. (D) Confirmation of the expression of HA protein in C-KCE-HA-infected CEF using immunofluorescence.