Figure 1

Effects of glutamine on viability of PK- 15 cells. PK-15 cells without (A) or with (B) PCV2 infection were grown in 96-well plates with DMEM containing 4 mM glutamine for 24 h until 50% confluence was reached. Subsequently, the medium was changed to fresh DMEM with various concentrations of glutamine (0–16 mM). The cells were incubated for an additional 48 h before the cell viability was determined by MTT assay. Values are given as mean ± SD from three independent experiments. Groups were compared by a 1-way ANOVA followed by a least-significant difference test (*P < 0.05, **P < 0.01).