Figure 5

Whole mount in situ hybridization of FhTLM in adult liver fluke. (A) Adult stage treated with antisense FhTLM probe showing a blue precipitate at several parts of the worm cephalic cone including oral sucker (OR), ventral sucker (VS), no staining detected in uterus (UT), oviduct (OV) and vitelline gland (VG). (B) Sense control showing unstained worm. Scale bar, A & B, indicates 3 mm. Localization of FhTLM transcript on the cephalic cone of the adult worms (C). Control worm with no visible signal (D). Scale bar, C & D, indicates 300 μm. NEJ stained showing extensive straining except in the pharynx, oral and ventral suckers (E). (F) control NEJs. Scale bar indicates 100 μm.