PCV2 vaccination induces IFN-γ/TNF-α co-producing T cells with a potential role in protection

Table 1 Antibodies for FCM staining

Antigen	Clone	Isotype	Fluorochrome	Labelling strategy	Source of primary Ab
CD4	74-12-4	IgG2b	Alexa488^a	Secondary antibody	In house
CD8α	76-2-11	IgG2a	PE-Cy7^b	Secondary antibody	In house
CD27	b30c7	IgG1	Alexa647^c	Directly conjugated	In house
IFN-γ	P2G10	IgG1	PE^d	Directly conjugated	BD Biosciences
TNF-α	MAb11	IgG1	BV605^e	Directly conjugated	BioLegend

^aAlexa488: Goat anti-Mouse IgG2b-Alexa488, Life Technologies, Carlsbad, CA, USA.
^bPE-Cy7: Goat anti-Mouse IgG2a-PE-Cy7, Southern Biotech, Birmingham, AL, USA.
^cAlexa647: Protein Labelling Kit Alexa 647, Life Technologies, Carlsbad, CA, USA.
^dPE, BD Biosciences, San Jose, CA, USA.
^eBrilliant Violet605, BioLegend, San Diego, CA, USA.

ISSN: 1297-9716