Skip to main content

Advertisement

Table 3 Identification of matched immunoreactive proteins of B. abortus that reacted with B. abortus -positive bovine antisera

From: Immunoproteomic identification of immunodominant antigens independent of the time of infection in Brucella abortus 2308-challenged cattle

Spot no. Gene name Gene ID a Protein identification Protein ID a Accession no a Sequence length Locus tag a Score M r p I Sequence coverage (%) Subcellular location c COG functional category d
          Experimental Theorectical b     
118 BruAb2_0325 3341905 aldehyde dehydrogenase YP_223118.1 Q579C7 500 BruAb2_0325 249 53744 53435 5.64 29 unknown G: Carbohydrate transport and metabolism
146 BruAb2_0024 3341776 branched chain amino-acid ABC transporter substrate-binding protein YP_222837.1 Q57A58 471 BruAb2_0024 438 50740 44322 6.43 25 periplasmic space E: Amino acid transport and metabolism
151 aspC 3339882 aspartate aminotransferase YP_222177.1 Q57C18 400 BruAb1_1488 193 43812 43554 5.94 18 unknown F: Nucleotide transport and metabolism
161 BruAb1_0775 3339474 3-hydroxyisobutyryl-CoA hydrolase YP_221504.1 Q57DZ1 349 BruAb1_0775 258 38120 37802 4.84 27 unknown H: Coenzyme transport and metabolism
162 tsf 3340671 elongation factor Ts YP_221867.1 Q57CX8 305 BruAb1_1167 367 32061 31491 5.03 35 cytoplasm J: Translation, ribosomal structure and biogenesis
164 mdh 3340925 malate dehydrogenase YP_222574.1 Q57AX1 320 BruAb1_1903 76 33854 33704 5.24 7 cytoplasm G: Carbohydrate transport and metabolism
169 tbpA 3340057 thiamine transporter substrate binding subunit YP_222421.1 Q57BC4 334 BruAb1_1742 310 36843 36752 6.06 19 periplasmic space H: Coenzyme transport and metabolism
178 BruAb1_1058 3341091 cysteine synthase A YP_221767.1 Q57D78 342 BruAb1_1058 417 34445 36701 5.94 30 cytoplasm E: Amino acid transport and metabolism
187 BruAb2_0291 3341871 hypothetical protein BruAb2_0291 YP_223086.1 Q579F9 330 BruAb2_0291 267 35457 35251 5.50 26 unknown R: General function prediction only
203 rpsB 3340672 30S ribosomal protein S2 YP_221868.1 Q57CX7 256 BruAb1_1168 259 29308 27999 5.88 29 ribosome J: Translation, ribosomal structure and biogenesis
204 ubiG 3340925 3-demethylubiquinone-9 3-methyltransferase YP_415219.1 Q2YLN5 248 BAB1_1875 410 27653 27486 5.79 31 cytoplasm E: Amino acid transport and metabolism
207 dapB 3341712 dihydrodipicolinate reductase YP_223731.1 Q576R4 268 BruAb2_0991 536 28792 27605 5.92 45 cytoplasm E: Amino acid transport and metabolism
218 BruAb2_0647 3342272 hypothetical protein BruAb2_0647 YP_223419.1 Q577X6 224 BruAb2_0647 302 24961 24805 4.83 34 unknown Q: Secondary metabolites biosynthesis, transport, and catabolism
227 BruAb2_0628 3342294 metal-dependent hydrolase YP_223400.1 Q577Z5 237 BruAb2_0628 511 25223 25124 5.58 51 cytoplasm R: General function prediction only
228 msrA 3341844 methionine sulfoxide reductase A YP_223747.1 Q576P8 218 BruAb2_1009 167 24230 24017 5.65 20 cytoplasm O: Posttranslational modification, protein turnover, chaperones
231 BruAb1_1470 3340810 50S ribosomal protein L25 YP_222213.1 Q57BY2 207 BruAb1_1470 171 22369 22383 5.91 47 ribosome J: Translation, ribosomal structure and biogenesis
239 BruAb1_0588 3339410 Fe-Mn superoxide dismutase YP_221327.1 Q57EG8 199 BruAb1_0588 359 22526 22540 5.83 37 unknown P: Inorganic ion transport and metabolism
240 rocF 3341875 arginase YP_223125.1 P0A2Y1 306 BruAb2_0333 188 33415 33182 5.63 24 unknown P: Inorganic ion transport and metabolism
243 gpm 3341713 phosphoglyceromutase YP_223732.1 Q576R3 206 BruAb2_0992 446 22929 22886 6.16 43 cytoplasm G: Carbohydrate transport and metabolism
253 secB 3339678 preprotein translocase subunit SecB YP_222709.1 P0C125 163 BruAb1_2047 343 17924 17878 4.89 46 cytoplasm O: Posttranslational modification, protein turnover, chaperones
254 ndk 3339959 nucleoside diphosphate kinase YP_221449.1 Q57E46 140 BruAb1_0713 58 15269 15278 5.27 20 cytoplasm F: Nucleotide transport and metabolism
257 BruAb2_0845 3341366 hypothetical protein BruAb2_0845 YP_223598.1 Q577E7 177 BruAb2_0845 500 18506 18517 5.02 43 unknown S: Function unknown
263 ohr 3341640 organic hydroperoxide resistance protein YP_223139.1 Q579A6 140 BruAb2_0347 8 14337 14232 5.63 13 unknown R: General function prediction only
  1. aGene ID, protein ID, accession no. and locus tag were retrieved from the NCBInr database.
  2. bTheoretical molecular weight from the UniProtKB database entry.
  3. cSubcellular locations were predicted using PSORTb v. 2.0.4.
  4. dCluster of orthologous groups (COG) protein database generated by comparing microbial genomes from the NCBI COG.