Immunoproteomic identification of immunodominant antigens independent of the time of infection in Brucella abortus 2308-challenged cattle

Table 1 Comparison of immunoreactive proteins of B. abortus after immune challenge in cattle

Antisera immunoreactions compared					No. of matched protein spots	Total no. of protein spots	Similarity (%) ^a
BP			NC	YP
Week 3	Week 7	Week 10
+^b	+	+	+	+	8	162	4.94
+	+	+	+	-	5	137	3.65
+	+	+	-	+	5	137	3.65
+	+	+	-	-	55	120	45.83
+	+	-	-	-	19	42	45.24
+	-	+	-	-	10	36	27.78
-^c	+	+	-	-	4	19	21.05

NC - negative control, YP - Y. enterocolitica-positive sera, BP - B. abortus-positive sera.
^aThe percent similarity was calculated as the number of proteins common to the compared antisera immunoreactions divided by the total number of proteins in these antisera immunoreactions × 100.
^bPositive reaction detected in immunoblotting.
^cNegative reaction detected in immunoblotting.